|
KMID : 1094720130180010144
|
|
Biotechnology and Bioprocess Engineering
2013 Volume.18 No. 1 p.144 ~ p.154
|
|
|
Differences in Caco-2 cell attachment, migration on collagen and fibronectin coated polyelectrolyte surfaces
|
|
Zanina N.
Mora L.
Othmane A.
Benard M.
Duncan A.
Jouenne T.
Vaudry D.
Souiri M.
|
|
|
Abstract
|
|
|
|
Metastasis mechanisms depend on cell metabolism changes, migration and adhesion to different tissues. To understand their choice of interaction site, the tumoral cell adhesion to model surfaces was studied. The response of Caco-2 tumoral cells cultured on polyelectrolyte film-functionalized surfaces with or without adhesion proteins (fibronectin or collagen IV) was analyzed. Using the layer-by- layer method, multilayer films were prepared with cationic poly(allylamine hydrochloride) and anionic poly(sodium 4-styrenesulfonate) polyelectrolytes. Film surface wettability was evaluated. The electrochemical impedance spectroscopy analyses were carried out to control the elaborated surfaces on which Caco-2 tumoral cells were cultured. The cell velocity was studied by video-microscopy and a cell colorimetric assay (WST-1) was used to quantify cell viability. The film surface parameters as well as the protein nature and localization in the film were found to modulate cell response. Results demonstrated that the cancer cell motility and proliferation were higher when cultured onto pure collagen located above the polyelectrolyte film and that the reverse surprisingly was observed when proteins were inserted into the polyelectrolyte film. Data also showed that cell motility was correlated with a high charge transfer resistance (Rct) and a low surface free energy (SFE) polar component (electron donor character). This relationship was valid only for pure external proteins. Thus, fibronectin exhibited a low Rct and a high SFE polar component, which decreased cell motility and proliferation.
|
|
|
KEYWORD
|
|
|
Caco-2 cells, polyelectrolyte multilayer, adhesion proteins, migration, electrochemical impedance spectroscopy
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|